Research

Cancer Diagnostics with Protein Markers

Development of low-cost, rapid analytical tools for cancer diagnostics can expedite early diagnosis and larger impact on integration of such technologies into clinical setting. Moreover, protein markers can be assessed as indicators of aggressive cancers, as they improve diagnosis and  subsequently treatment of such cancers. Specifically, potential protein markers in serum for detection of oral cancer and bladder cancer has been investigated.

References:
1. In preparation: G.S. Phadke, J.E. Satterwhite-Warden, D. Choudhary, J.A. Taylor, and J.F. Rusling, “Online Microfluidic Protein Capture Electrochemical Assay of CD62L for the Assessment of Metastasis in Bladder Cancer”.
2. C.E. Krause, B.A. Otieno, G.W. Bishop, G.S. Phadke, L. Choquette, R.V. Lalla, D.E. Peterson, and J.F. Rusling (2015), “Ultrasensitive microfluidic array for serum proinflammatory cytokines and C-reactive protein to assess oral mucositis risk in cancer patients”, Anal. Bioanal. Chem., 407: 7239-7243. Link

2D Deposition of Nanomaterials

Nanomaterials, with the diversity in their chemical identities, shapes, and sizes, display a wide variety of optical, electrical, and catalytic properties. The flexibility provided by chemical deposition of these materials, with highly distinct print patterns, can boost applications to a broad range of fields. Current efforts are  focused on developing anti-fraud technology, multiplexed array for assessment of catalytic activity, product of electrochemical arrays.  

References:
1. http://www.fujifilmusa.com/products/industrial_inkjet_printheads/deposition-products/dmp-2800/

Small Biomolecules Disease Markers

Small molecules, such as amino acids, modified amino acids, and other biological components, can signal disease conditions. Matrix-assisted laser desorption/ ionization mass spectrometry or MALDI-MS, a traditionally qualitative technique, can be extended to quantitative analysis of such small biomolecules. The rapid, low-cost, and automated analysis, provided by adoption of MALDI-MS, can provide for newer clinical tests with significantly wider biomolecule panels.

References:
1. N. Bhattacharya, A. Singh, A. Ghanate, G. Phadke, D. Parmar, D. Dhaware, T. Basak, S. Sengupta, and V. Panchagnula (2014), “Matrix-assisted laser desorption/ ionization mass spectrometry analysis of dimethyl arginine isomers from urine”, Anal. Methods, 6: 4602-4609. Link

Development of Quantitative Analytical Software

MALDI-MS and other mass spectrometric techniques often come with licensed and individualized analytical software. However, current software for MALDI-MS do not include a quantitative modules. The in-house software, developed at the Panchagnula lab, includes an open-access file format, isotope matching, and quantitative analysis and visualization formats.

References:
1. In preparation: A.V. Ghanate, D. Parmar, N. Bhattacharya, G. Phadke, and V. Panchagnula, “MQ: Open source platform for quantitative HRMS based Metabolomics analysis”.

2. http://www.ldi-ms.com/services/software

Quantitative Analysis of Pesticide

Quantitative MALDI-MS analysis of pesticides can provide a greener alternative to current analytical methodologies for pesticides. Pesticide assessment is of particular importance in the field of environmental and food testing.

References:
1. V. Mahale, A. Singh, G.S. Phadke, A. D. Ghanate, D.P. Oulkar, K. Banerjee, V. Panchagnula (2017), “Determination of Triazines and Triazoles in grapes using atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry”, J. AOAC Int., DOI: https://doi.org/10.5740/jaoacint.17-0047. Link